Method of producing natural killer cells and composition for treating cancer
AssigneeNKMAX Co., Ltd.
InventorsSang Woo Park, Yong Man Kim, Jae Seob Jung, Yong-Hee Rhee
Life of this patent
Estimating the term…
Abstract
A method for producing natural killer cells is disclosed. The method comprises isolating peripheral blood mononuclear cells (PBMCs) from a blood sample; isolating at least one of CD56+ cells and/or CD3−/CD56+ cells from the PBMCs; and co-culturing the at least one of CD56+ cells and/or CD3−/CD56+ cells with a combination of feeder cells in the presence of a cytokine. A composition for treating cancer is also disclosed. The composition comprises the CD56+ natural killer cells produced by the disclosed method and a cytokine.
Claims18
A method of expanding natural killer cells in culture, comprising:
- isolating peripheral blood mononuclear cells (PBMCs) from a blood sample;
- isolating at least one of CD56+ cells and/or CD3−/CD56+ cells from the PBMCs;
- and
- co-culturing the at least one of CD56+ cells and/or CD3−/CD56+ cells with a combination of feeder cells in the presence of at least two cytokines;
- wherein the combination of feeder cells comprises irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells,
- wherein the at least two cytokines comprise IL-2 and IL-21.
A method of expanding natural killer cells in culture, comprising:
- isolating peripheral blood mononuclear cells (PBMCs) from a blood sample;
- isolating at least one of CD56+ cells and/or CD3−/CD56+ cells from the PBMCs;
- and
- co-culturing the at least one of CD56+ cells and/or CD3−/CD56+ cells with a combination of feeder cells in the presence of at least two cytokines;
- wherein the combination of feeder cells comprises irradiated Jurkat cells and irradiated Epstein-Barr virus transformed lymphocyte continuous line (EBV-LCL) cells,
- wherein the at least two cytokines comprise IL-2 and IL-21, and
- wherein both the feeder cells and the cytokines are supplemented at intervals throughout the expansion in culture.
Dependencies are inferred from the claim text.