Luterial and method for isolating and culturing the same

A method for isolating luterial from body fluid, comprising:

• (A) conducting at least one of the following with a body fluid:
• (i) obtaining the body fluid by collecting a supernatant after centrifuging of the body fluid to remove platelet and blood-derived substances having a size greater than that of platelet;
• (ii) filtering the body fluid with filters of various pore sizes to select for particles within a specific size range;
• (iii) centrifuging the body fluid at 100,000 g-200,000 g to collect the supernatant;
• (B) conducting at least one of the following with a solution obtained from (A):
• (i) irradiating the solution with visible light to select for autofluorescent and mobile particles;
• or
• (ii) subjecting the solution to a density gradient fractionation to further separate the particles in the solution;
• and
• (C) recovering luterial from fluids obtained from conducting (A) and (B) by isolating a particle having one or more of the following characteristics:
• (a) a positive staining reaction with Janus green B, Acridine Orange, DAPI, or Rhodamine 123 in a fluorescence test;
• (b) in healthy condition expressing beta-proteobacteria-derived and gamma-proteobacteria-derived genes and having a size of less than 800 nm;
• (c) in a diseased condition, expressing not only beta-proteobacteria or gamma-proteobacteria-derived genes, but also eukaryote Streptophyta genes and having a size of greater than 800 nm;
• (d) exhibiting ATP production in normal conditions;
• (e) a cell or cell-like structure completely different from mitochondria or exosomes;
• (f) circular or oval in shape in a healthy condition, and non-uniform in morphology in a diseased condition;
• (g) containing a membrane structure and is adherent;
• (h) presence inside or outside cells;
• (i) exhibiting mobility and undergoing fusion and/or fission events;
• (j) mutant luterial bursting in a certain condition and having stemness after bursting;
• (k) a function of regulating p53 gene and telomeres;
• (l) expressing at least one protein selected from the group consisting of CD14, CD24, CD29, CD34, CD39, CD44, CD45 (CD45RA/CD45RO), CD73, CD90, CD105, CD133, CD173, CD326, CD332, OCT4, ND1 (OXPHOS complex I), CO1 (OXPHOS complex IV), ATP6 (OXPHOS complex V), ATP8 (OXPHOS complex V), RNR1, RNR2 and 7S;
• and
• (m) presence in a fraction with 0.99 g/ml or less density in 15-60% sucrose density gradient.