Methods for differentiating pluripotent cells
AssigneeFUJIFILM Cellular Dynamics, Inc.
InventorsChristopher W. McMahon, Lauren E. Little, Wen Bo Wang, Nathaniel A. Elliott
Life of this patent
Estimating the term…
Abstract
Methods are provided, in some aspects, for differentiating pluripotent cells into midbrain dopaminergic (DA) neurons using a mono-SMAD inhibition or inhibition of SMAD signaling with only one SMAD inhibitor. In some embodiments, mono-SMAD inhibition utilizes a single inhibitor of bone morphogenic protein (BMP) for differentiating pluripotent cells into midbrain DA neurons.
Claims62
An in vitro method for preparing a cell composition comprising human cells that express both forkhead box protein A2 (FOXA2) and LIM homeobox transcription factor 1 (LMX1) (FOXA2+/LMX1+ cells) comprising culturing human pluripotent cells in the presence of the following signaling modulators:
- (a) a single inhibitor of Small Mothers Against Decapentaplegic (SMAD) signaling,
- (b) at least one activator of Sonic hedgehog (SHH) signaling, and
- (c) at least one activator of wingless (Wnt) signaling;
- and culturing said cells in the presence of said modulators for a period of time sufficient to provide a cell composition comprising said FOXA2+/LMX1+ cells;
- wherein the culturing does not comprise culturing the human pluripotent cells in the presence of a second inhibitor of Small Mothers Against Decapentaplegic (SMAD) signaling.
Dependencies are inferred from the claim text.