Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor
AssigneeTechnion Research & Development Foundation Limited
InventorsMichal Amit, Joseph Itskovitz-Eldor
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Abstract
The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
Claims19
A method of establishing a human pluripotent stem cell line capable of being maintained in an undifferentiated, pluripotent and proliferative state, the method comprising:
- culturing human pluripotent stem cells under culturing conditions devoid of feeder cells and including a matrix and a culture medium supplemented with TGFβ1, bFGF and/or LIF to thereby obtain the human pluripotent stem cell line.
A xeno-free, feeder cells-free culture system comprising a matrix and a culture medium, wherein said culture medium comprises TGFβ1, bFGF and LIF.
A culture system comprising a matrix and a tissue culture medium, wherein said matrix is selected from the group consisting of s a fibronectin matrix, a laminin matrix, foreskin fibroblast matrix, a synthetic fibronectin matrix, a mouse embryonic fibroblast (MEF) matrix and a basement membrane matrix extracted from Engelbreth-Holm-Swarm (EHS) mouse sarcoma, wherein said culture medium comprises TGFβ1, bFGF and LIF, wherein the culture system being capable of maintaining human pluripotent stem cells cultured therein in a proliferative, pluripotent and undifferentiated state.
A culture system comprising a matrix and a tissue culture medium, wherein said matrix is a laminin matrix, wherein said culture medium comprises TGFβ1, bFGF and/or LIF, wherein the culture system being capable of maintaining human pluripotent stem cells cultured therein in a proliferative, pluripotent and undifferentiated state.
Dependencies are inferred from the claim text.