Composition for collecting and preserving placental stem cells and methods of using the composition

A method of isolating stem cells from a population of cells with which the stem cells are normally associated with in an intact organ, comprising contacting said stem cells with a solution comprising a caspase inhibitor, a JNK inhibitor and a protease,

• wherein the JNK inhibitor is an inhibitor of apoptosis, said JNK inhibitor is represented by the structure
• wherein:
• R1 is aryl or heteroaryl optionally substituted with one to four substituents independently selected from R7;
• R2 is hydrogen;
• R3 is hydrogen or lower alkyl;
• R4 represents one to four optional substituents, wherein each substituent is the same or different and independently selected from halogen, hydroxy, lower alkyl and lower alkoxy;
• R5 and R6 are the same or different and independently —R8, —(CH2)aC(═O)R9—, —(CH2)aC(═O)OR9, —(CH2)aC(═O)NR9R10, —(CH2)aC(═O)NR9(CH2)bC(═O)R10, —(CH2)aNR9C(═O)R10, (CH2)aNR11C(═O)NR9R10, —(CH2)aNR9R10, —(CH2)aOR9, —(CH2)aSOcR9 or —(CH2)aSO2NR9R10;
• or R5 and R6 taken together with the nitrogen atom to which they are attached to form a heterocycle or substituted heterocycle;
• R7 is at each occurrence independently halogen, hydroxy, cyano, nitro, carboxy, alkyl, alkoxy, haloalkyl, acyloxy, thioalkyl, sulfinylalkyl, sulfonylalkyl, hydroxyalkyl, aryl, arylalkyl, heterocycle, substituted heterocycle, heterocycloalkyl, —C(═O)OR8, —OC(═O)R8, —C(═O)NR8R9, C(═O)NR8OR9, —SOcR8, —SOcNR8R9, —NR8SOcR9, —NR8R9, —NR8C(═O)R9, —NR8C(═O)(CH2)bOR9, —NR8C(═O)(CH2)bR9, —O(CH2)bNR8R9, or heterocycle fused to phenyl:
• R8, R9, R10 and R11 are the same or different and at each occurrence independently hydrogen, alkyl, aryl, arylalkyl, heterocycle, heterocycloalkyl;
• or R8 and R9 taken together with the atom or atoms to which they are attached to form a heterocycle;
• a and b are the same or different and at each occurrence independently selected from 0, 1, 2, 3 or 4;
• and
• c is at each occurrence 0, 1 or 2;
• or a pharmaceutically acceptable salt, hydrate, solvate, clathrate, enantiomer, diastereomer, racemate, or mixture of stereoisomers thereof.