Cell separation chip and method for separating cells using same

A cell separation chip for separating cells from a solution containing the cells, comprising:

• an upper plate;
• a membrane positioned on a predetermined region of the upper plate;
• an absorption device positioned at a lower end of the upper plate so as to absorb a solution which has penetrated the membrane from the solution containing the cells, and
• a lower plate positioned at the lower end of the upper plate by being connected to the absorption device,
• wherein the membrane comprises:
• an upper end region forming an upper end of the membrane as a region where the solution containing cells is contacted first, wherein the upper end region has a structure configured that cells from the solution containing cells are left on the upper end region of the membrane, the upper end region having a hole with a diameter of 2 μm or smaller;
• and
• a lower end region forming a lower end of the membrane as a region where the solution is contacted with the absorption device and the remainder of the solution are passed through the membrane and absorbed by the absorption means positioned at a lower end of the membrane, the lower end region having a hole with a diameter of 10 μm or greater.