Automatic multi-tray and multi-plate bioreactor systems for adherent cultures

A bioreactor system comprising:

• a closed culture vessel comprising:
• a multi-tray culture vessel comprising at least two rectangular cell culture compartments having a lid, a bottom, two sides and two ends, arranged in a stacked orientation with respect to each other;
• and configured to allow cells to attach and grow as a monolayer on the bottom surface;
• at least one port for medium or gas in and out of the vessel, wherein each port is in gas/fluid communication among all compartments through a gas/fluid flow pathway for allowing gas/fluid to flow through a manifold and into each culture compartment;
• a longitudinal supporting vessel comprising:
• a tube positioned to lie along a longitudinal axis of the tube, the tube having ends;
• at least one end opening to allow culture material or gas to flow into or out of the tube and the ends;
• a platform is configured to hold, secure and manipulate the at least one closed culture vessel;
• wherein the platform is a seesaw platform swung along the axis perpendicular to the pivot with the tilting angle of less than 90 degrees in each side of the pivot, wherein the pivot is secured on the tube;
• and a driving and rotating assembly configured to rotate and change the position of the supporting vessel and the culture vessel about the longitudinal axis of the tube at an angle position of less than 360 degrees and a holding time, wherein the axis is perpendicular to the first axis swung by the seesaw platform.