Systems and methods for biomimetic fluid processing

A biomimetic microfluidic system comprising:

• a substrate;
• a first channel formed in the substrate, the first channel extending from a first input to a first output along a longitudinal direction and extending along a first transverse direction;
• a second channel formed in the substrate, the second channel extending from a second input to a second output along the longitudinal direction and extending along the first transverse direction, wherein the first and second channels extend substantially parallel for a longitudinal dimension and are separated by columns extending along a second transverse direction;
• a series of apertures formed in between the columns to create a fluid communication path passing between the channels, wherein each of the series of apertures is positioned proximal to a first portion of the substrate, extends along the longitudinal direction further than along the first transverse direction and the second transverse direction, and is configured to selectively capture a biological source material from a fluid passing therethrough;
• a first source connected to the first input, the first source being configured to selectively introduce into the first channel at least one first biological composition comprising the biological source material at a first channel flow rate;
• and
• a second source connected to the second input, the second source being configured to selectively introduce into the second channel at least one second biological composition at a second channel flow rate,
• wherein the first source and the second source are configured to control the first channel flow rate and the second channel flow rate, respectively, to create a differential between the channels to generate physiological shear rates in the second channel that are sufficient to induce the captured biological source material to produce a target biological substance, and
• wherein the system further includes a first flow filter positioned upstream from the first input, and a second flow filter positioned upstream from the second input, wherein upstream is defined relative to a direction of flow for the at least one first biological composition and the at least one second biological composition.