Protease variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsMiguel Duarte Guilherme Pereira Toscano, Esben Peter Friis, Bena-Marie Lue
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Abstract
The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims16
A protease variant comprising one or more alterations selected from the group consisting of A1L, A1*, V2I, P3*, S4*, S4C, Q6C, Q18A, E50Q, N59L, L61K, V62G, G64A, G64S, S65F, Q70T, Q70L, Q70N Q70Y, Q70F, Q70M, Q70A, Q70V, Q97K, G107N, *108aA, N109T, *109aA, N109A, S111I, S111R, Y113S, S114V, S114A, S114F, D126G, D126K, E127R, S133H, I137N, M139L, S143R, S144P, S144R, A145E, L149R, L149T, I150L, I150T, I150S, I150V, V162S, V162E, V162Q, V162G, V162F, V162T, V162Y, I164M, S171D, G182S, Q198E, Q198D, G200C, G200S, A233S, T246M, S274V, Q275Y, L276I and T297P, wherein positions correspond to the positions of SEQ ID NO:
- 3, and wherein the protease variant has a sequence identity to SEQ ID NO:
- 3 of at least 75% and the variant has protease activity.
A method for obtaining a protease variant, comprising introducing into a parent protease one or more of the following alterations:
- A1L, A1*, V2I, P3*, S4*, S4C, Q6C, Q18A, E50Q, N59L, L61K, V62G, G64A, G64S, S65F, Q70T, Q70L, Q70N Q70Y, Q70F, Q70M, Q70A, Q70V, Q97K, G107N, *108aA, N109T, *109aA, N109A, S111I, S111R, Y113S, S114V, S114A, S114F, D126G, D126K, E127R, S133H, I137N, M139L, S143R, S144P, S144R, A145E, L149R, L149T, I150L, I150T, I150S, I150V, V162S, V162E, V162Q, V162G, V162F, V162T, V162Y, I164M, S171D, G182S, Q198E, Q198D, G200C, G200S, A233S, T246M, S274V, Q275Y, and L276I of SEQ ID NO 3, wherein the variant has an amino acid sequence which is at least 75% identical to SEQ ID NO:
- 3, and recovering the variant.
Dependencies are inferred from the claim text.