Immunoglobulin purification
AssigneeHoffmann-La Roche Inc.
InventorsRoberto Falkenstein, Claudia Giessel
Life of this patent
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Abstract
The current invention reports a method for purifying an immunoglobulin, wherein the method comprises applying an aqueous, buffered solution comprising an immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby the immunoglobulin in monomeric form does not bind to the cation exchange material, and recovering the immunoglobulin in monomeric form from the solution after the contact with the cation exchange material.
Claims24
A method for obtaining an immunoglobulin in monomeric form from a solution comprising the immunoglobulin in monomeric and in aggregated form, characterized in that said method comprises the following step:
- a) applying an aqueous, buffered solution comprising said immunoglobulin in monomeric and in aggregated form to a cation exchange material under conditions whereby said immunoglobulin in monomeric form does not bind to said cation exchange material, and recovering said immunoglobulin in monomeric form from said solution after the contact with said cation exchange material,
- wherein said aggregated form is an immunoglobulin molecule associated covalently or non-covalently with at least one additional immunoglobulin molecule, and wherein said cation exchange material is the sole chromatography material in step a).
Dependencies are inferred from the claim text.