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US 10,590,181 B2Granted
Mutant channelrhodopsins with altered ion selectivity
AssigneeMassachusetts Institute of Technology
InventorsEdward S. Boyden, Yongku Peter Cho, Nathan C. Klapoetke, Amy S. Chuong, Fei Chen
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Abstract
The invention, in some aspects relates to compositions and methods for altering cell activity and function and the introduction and use of mutant light-activated ion channels and variants thereof.
Claims19
A light-activated ion channel polypeptide comprising an amino acid sequence set forth as (i) SEQ ID NO:
- 11 or (ii) the amino acid sequence of SEQ ID NO:
- 11 with one or more amino acid modifications wherein the amino acids corresponding to positions S71, A90, and G114 of SEQ ID NO:
- 11 are not modified, and having at least 98% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
- 11, wherein the light-activated ion channel polypeptide comprises a seven-transmembrane-domain region.
1 more element
A light-activated ion channel polypeptide comprising an amino acid sequence set forth as (i) SEQ ID NO:
- 11 or (ii) the amino acid sequence of SEQ ID NO:
- 11 with one or more amino acid modifications wherein the amino acids corresponding to positions S71, A90, and G114 of SEQ ID NO:
- 11 are not modified, and having at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
- 11, wherein the light-activated ion channel polypeptide comprises a seven-transmembrane-domain region and wherein the light-activated ion channel polypeptide has at least one of a lower level of an ion flux and a lower level of proton flux compared to a control level of the ion flux and proton flux, respectively, when expressed in a cell membrane and contacted with an ion-channel-polypeptide-activating light under suitable conditions for the ion flux and proton flux through the expressed light-activated ion channel polypeptide.
1 more element
Dependencies are inferred from the claim text.