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US 10,590,176 B2Granted
Engineered programmable molecular scaffolds from porous protein crystals
AssigneeColorado State University Research Foundation
InventorsChristopher D. Snow, Thaddaus R. Huber
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Abstract
The present disclosure provides compositions and methods for preparing engineered porous protein crystals comprising at least one guest molecule.
Claims46
A composition comprising an engineered porous protein crystal and at least one guest molecule,
- wherein the engineered porous protein crystal comprises:
- at least one pore having a diameter equal to or greater than 3 nm wherein the at least one pore's diameter is large enough to permit entry of the entirety of at least one guest molecule into the pore;
- and
- at least one binding site for the at least one guest molecule within the interior of the at least one pore, and
- wherein the at least one guest molecule comprises a metal nanoparticle, a biomacromolecule, or a combination thereof, and is bound to the engineered porous protein crystal at the at least one binding site.
2 more elements
A method for preparing a porous protein crystal guest molecule conjugate, the method comprising:
- obtaining a porous protein crystal, wherein the porous protein crystal comprises at least one pore and at least one binding site for the at least one guest molecule within the interior of the at least one pore, wherein the at least one pore's diameter is large enough to permit entry of the entirety of at least one guest molecule into the pore, and wherein the porous protein crystal has been reacted with a crosslinking agent to produce a crosslinked porous protein crystal and the crosslinking agent bonds adjacent monomers of the porous protein crystal, wherein the crosslinking agent comprises at least one functional group selected from N-hydroxysuccinimide (NHS ester), imidoester, maleimide, pyridyldithiol, carbodiimide, aldehyde, and combinations thereof, and wherein the at least one guest molecule comprises a metal nanoparticle, a biomacromolecule, or a combination thereof;
- and
- incubating the crosslinked porous protein crystal with at least one guest molecule to produce a porous protein crystal guest molecule conjugate.
A method for determining the molecular structure of at least one guest molecule conjugated to a porous protein crystal, the method comprising:
- (a) obtaining a porous protein crystal guest molecule conjugate, wherein the porous protein crystal guest molecule conjugate comprises a guest molecule, wherein the guest molecule comprises a metal nanoparticle, biomacromolecule, or a combination thereof, and a porous protein crystal, wherein the porous protein crystal comprises:
- at least one pore having a diameter equal to or greater than 3 nm wherein the at least one pore's diameter is large enough to permit entry of the entirety of at least one guest molecule into the pore;
- and
- at least one binding site for the at least one guest molecule within the interior of the at least one pore,
- wherein the porous protein crystal has been reacted with a crosslinking agent to produce a crosslinked porous protein crystal and the crosslinking agent crosslinks adjacent monomers of the porous protein crystal;
- (b) imaging the porous protein crystal guest molecule conjugate;
- and
- (c) determining the molecular structure of the at least one guest molecule.
5 more elements
A method for preparing a porous protein crystal guest molecule conjugate, the method comprising:
- obtaining a porous protein crystal, wherein the porous protein crystal comprises at least one pore and at least one binding site for the at least one guest molecule within the interior of the at least one pore, wherein the at least one pore's diameter is large enough to permit entry of the entirety of at least one guest molecule into the pore;
- reacting the porous protein crystal with a crosslinking agent to produce a crosslinked porous protein crystal, wherein the crosslinking agent bonds adjacent monomers of the porous protein crystal, and wherein the crosslinking agent comprises at least one functional group selected from N-hydroxysuccinimide (NHS ester), imidoester, maleimide, pyridyldithiol, carbodiimide, aldehyde, and combinations thereof, and;
- incubating the crosslinked porous protein crystal with at least one guest molecule to produce a porous protein crystal guest molecule conjugate, wherein the at least one guest molecule comprises a metal nanoparticle, a biomacromolecule, or a combination thereof.
Dependencies are inferred from the claim text.