Modular DNA-binding domains and methods of use
OwnerUlla Bonas, Jens Boch +2inventors
InventorsUlla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
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Abstract
The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.
Claims9
An RNA encoding a fusion protein, the fusion protein comprising a transcription activator-like (TAL) effector repeat domain that is capable of selectively recognizing a target nucleotide sequence and a catalytic domain comprising an activity that is capable of modifying DNA, wherein the repeat domain comprises contiguous repeat units and wherein the order of the contiguous repeat units in the repeat domain correlates with the linear sequence of bases in the target nucleotide sequence.
Dependencies are inferred from the claim text.