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US 10,590,164 B2Granted
Method for preparing a composition comprising highly concentrated antibodies by ultrafiltration
AssigneesChugai Seiyaku Kabushiki Kaisha, Genentech, Inc.
InventorsKelby Lau, Jean Bender, Saeko Tanaka, Rumiko Wakayama, Hidenari Yamada, Tomonori Isoda, Masayoshi Oh-Eda
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Abstract
The present invention provides a method for preparing a composition comprising highly concentrated antibodies by ultrafiltration in batch concentration mode having a first constant feed rate step and a second controlled feed rate step.
Claims2
A method for preparing a composition comprising concentrated proteins by ultrafiltration, wherein the method comprises concentrating a protein preparation by ultrafiltrating the preparation, said ultrafiltrating comprising:
- a) regulating a feed flow rate of the preparation to allow a value of feed pressure applied to an ultrafiltration membrane to increase to 3.5 bar or a value of above 3.5 bar;
- and
- b) decreasing the feed flow rate to maintain or decrease the value of the feed pressure applied to the ultrafiltration membrane after the step (a),
- wherein the protein preparation directly after said ultrafiltrating has a protein concentration above 200 g/L or a viscosity above 10 mPa·s.
1 more element
Dependencies are inferred from the claim text.