Lincomycin biosynthetic intermediates, method for preparation, and use thereof
AssigneesShanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Huzhou Center of Bio-Synthetic Innovation, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
InventorsWen Liu, Min Wang, Dongxiao Xu, Qunfei Zhao, Qinglin Zhang
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Abstract
Provided in the present invention are a class of Lincomycin biosynthetic intermediates and a preparation method and use thereof. Specifically provided are Lincomycin biosynthetic intermediates obtained from the genetic modification of a Lincomycin producing bacterium, and a method for the production thereof through fermentation and purification through separation.
Claims3
A microbial strain, wherein the microbial is Streptomycin lincolnensis, and in the strain, one or more genes selected from a group consisting of the following have been inactivated or knocked out:
- lmbE, lmbE3457, lmbV, mshA, mshC, lmbC, lmbD, lmbN, and lmbF.
A preparation method for a compound, wherein the method comprises:
- (a) fermenting a microbial strain, wherein the microbial is Streptomycin lincolnensis, and in the microbial strain, one or more genes selected from the group consisting of the following have been inactivated or knocked out:
- lmbE, lmbE3457, lmbV, mshA, mshC, lmbD, lmbN, and lmbF to obtain a fermentation product containing the compound;
- and
- (b) isolating the compound from the fermentation product;
- and optionally converting the compound into a pharmaceutically acceptable salt thereof,
- wherein the compound is represented by formula I:
- wherein R1 is selected from a group consisting of H, halogen, C1-8alkyl, C2-8 alkenyl, C2-8 alkynyl, and
- where R8 is selected from a group consisting of H, halogen and C1-8 alkyl;
- and
- R2 is selected from
Dependencies are inferred from the claim text.