System for portable and easy-to-use detection of analytes with mobile computing device

A system for identifying a presence, absence, and/or quantity of a molecule of interest in a sample, the system comprising:

• a cartridge comprising:
• a first reservoir that receives the sample collected by a sample collection device to form a fluid mixture therein, the fluid mixture comprising the sample and signaling enzymes,
• a second reservoir that holds a fluid comprising chemical substrates,
• an analysis channel configured to receive the fluid mixture from the first reservoir and the fluid from the second reservoir, and
• an electrochemical sensor disposed within the analysis channel to be exposed to the fluid mixture and then to the fluid, the electrochemical sensor configured to sense electrons upon mixing of the signaling enzymes localized over the electrochemical sensor from the fluid mixture and the chemical substrates from the fluid, the sensed electrons indicative of the presence, absence, and/or quantity of the molecule of interest in the sample;
• and
• a reader configured to receive the cartridge, the reader comprising:
• a pressure sensor configured to be operatively coupled to the first reservoir when the cartridge is inserted into the reader, the pressure sensor configured to sense a flex in the first reservoir due to insertion of the sample collection device into the cartridge and generate a pressure signal, the reader configured to begin a testing protocol in response to the pressure signal.