Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases

A method for modulating the immune system in a subject, comprising subcutaneously administering to a subject immune globulin (IG) and a soluble hyaluronidase to enhance the immune system of the subject, wherein:

• the soluble hyaluronidase is a soluble neutral active human hyaluronidase glycoprotein (sHASEGP):
• the hyaluronidase is active at neutral pH and contains at least one sugar moiety that is covalently attached to an asparagine (N) residue of the hyaluronidase polypeptide:
• the hyaluronidase polypeptide does not comprise the complete sequence of amino acids set forth in SEQ ID NO:
• 1;
• and the hyaluronidase polypeptide consists of:
• (i) a contiguous sequence of amino acid residues contained within SEQ ID NO:
• 1 that includes at least amino acids 36-464 of SEQ ID NO:
• 1, wherein the polypeptide is truncated within the C-terminus of SEQ ID NO:
• 1 at a C-terminal amino acid residue selected from among 477, 478, 479, 480, 481, 482, 483 and 494 of SEQ ID NO:
• 1;
• or
• (ii) a sequence of amino acid residues that has at least 95% amino acid sequence identity with a sequence of amino acids set forth in (i).