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US 10,588,972 B2Granted
Phthalocyanine probes and uses thereof
AssigneeLI-COR, Inc.
InventorJoy L. Kovar
Life of this patent
Estimating the term…
Abstract
The present invention relates to compositions and methods for destroying target cells in a patient using photodynamic therapy. In particular, the present invention provides a photosensitizing agent based on a small molecular weight (<50 kDa) protein or peptide or a small molecule that is conjugated to a phthalocyanine dye, such as IRDye® 700DX.
Claims17
A method for inducing cytotoxicity by apoptosis in a subject having a disease or condition, the method comprising:
- (a) administering to the subject a therapeutically effective agent comprising a phthalocyanine dye, having a wavelength of 660 to 740 nm, conjugated to a probe that specifically binds to a cell, wherein the bound phthalocyanine dye conjugated probe is internalized into the cell, wherein the probe has a molecular weight of less than 10 kDa and is selected from the group consisting of a peptide, and a small molecule;
- and
- (b) within 20 minutes to allow internalization of the conjugated probe dye, having a wavelength of 660 to 740 nm, irradiating said cell with an appropriate excitation light in an amount to effectively induce cell death predominately by apoptosis.
A method for treating a solid tumor in a subject having cancer, the method comprising:
- (a) administering to the subject a therapeutically effective agent comprising a phthalocyanine dye , having a wavelength of 660 to 740 nm, conjugated to a probe that specifically binds to a cell of the solid tumor, wherein the bound phthalocyanine dye conjugated probe is internalized into the cell, wherein the probe has a molecular weight of less than 10 kDa and is selected from the group consisting of a peptide, and a small molecule;
- and
- (b) within 20 minutes to allow internalization of the conjugated probe dye, having a wavelength of 660 to 740 nm, irradiating the solid tumor with an appropriate excitation light in an amount to effectively reduce the size of the solid tumor, by inducing cell death predominately by apoptosis.
Dependencies are inferred from the claim text.