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US 10,588,861 B2Granted
Crosslinked polysaccharide beads and their biomedical uses
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Universite Paris Diderot—Paris 7
InventorsDidier Letourneur, Catherine Le Visage, Sidi Mohammed Derkaoui
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Abstract
The present inventions relates to beads as biocompatible material adapted for use within the human or animal body. Said beads are highly useful for tissue engineering, in situ tissue regeneration, as well as for drug and/or cells delivery. In addition, said beads may support biotechnological applications such as cell carriers.
Claims10
A crosslinked polysaccharide bead having a spherical or ovoid shape obtained by
- a) preparing an alkaline aqueous solution comprising at least two polysaccharides and a cross linking agent;
- b) in the absence of surfactant, dispersing said alkaline aqueous solution into an hydrophobic phase consisting of a vegetal oil, in order to obtain a water-in-oil (w/o) emulsion;
- and
- c) transforming the w/o emulsion into beads by placing said w/o emulsion at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said at least two polysaccharides;
- wherein said polysaccharides are selected from mixtures of dextran and pullulan, or mixtures of dextran, pullulan and fucoidan,
- wherein said crosslinked polysaccharide bead is free of any organic solvent and surfactant, and
- wherein said crosslinked polysaccharide bead has a mean diameter of more than 10 μm.
4 more elements
Dependencies are inferred from the claim text.