Biomedical patches with aligned fibers

A three-dimensional electrospun nanofiber scaffold for use in repairing a defect in a tissue substrate, the three-dimensional electrospun nanofiber scaffold comprising:

• a flexible deposited electrospun fiber network of varying density, the deposited electrospun fiber network comprising:
• a first set of deposited electrospun fibers comprising a first bioresorbable polymer, wherein the first bioresorbable polymer comprises glycolic acid;
• and
• a second set of deposited electrospun fibers comprising a second bioresorbable polymer, the second set of deposited electrospun fibers coupled to the first set of deposited electrospun fibers,
• wherein the first bioresorbable polymer comprises a different composition from the second bioresorbable polymer,
• wherein at least a first portion of the flexible deposited electrospun fiber network comprises a higher density of fibers than a second portion of the flexible deposited electrospun fiber network, and wherein the first portion comprises a higher tensile strength than the second portion;
• wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be conformable to the defect in the tissue substrate,
• wherein the three-dimensional electrospun nanofiber scaffold comprises varying density to be sufficiently flexible to enable movement of the three-dimensional electrospun nanofiber scaffold by the tissue substrate,
• wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are configured to separate from each other within three months after application to the tissue substrate, and
• wherein each fiber of the deposited electrospun fiber network comprises a diameter of 1-1000 nanometers.

A monolayer electrospun nanofiber patch for use in repairing a defect in a tissue substrate, the monolayer electrospun nanofiber patch comprising:

• a flexible deposited electrospun fiber network of varying density, the deposited electrospun fiber network comprising:
• a first set of deposited electrospun fibers comprising a first bioresorbable polymer, wherein the first bioresorbable polymer comprises glycolic acid;
• and
• a second set of deposited electrospun fibers comprising a second bioresorbable polymer, the second set of deposited electrospun fibers coupled to the first set of deposited electrospun fibers,
• wherein the first bioresorbable polymer comprises a different composition from the second bioresorbable polymer,
• wherein an inner portion of the monolayer electrospun nanofiber patch comprises a different fiber density than an outer portion of the monolayer electrospun nanofiber patch;
• wherein the monolayer electrospun nanofiber patch comprises varying density to be conformable to the defect in the tissue substrate,
• wherein the monolayer electrospun nanofiber patch comprises varying density to be sufficiently flexible to enable movement of the monolayer electrospun nanofiber patch by the tissue substrate,
• wherein the first set of deposited electrospun fibers and the second set of deposited electrospun fibers are configured to degrade within three months after application to the tissue substrate, and
• wherein each fiber of the deposited electrospun fiber network comprises a diameter of 1-1000 nanometers.