Method and kit for diagnosing and treating neoplastic tissue

A method for practicing the diagnosis and treatment of tissue exhibiting intraepithelial neoplasia, the method comprising:

• providing a medical kit comprising:
• a speculum, a light source, a first pre-filled container containing saline solution, a second pre-filled container containing acetic acid, and a third pre-filled container containing fluorescein sodium;
• diagnosing the cervical intraepithelial neoplasia, the diagnosing comprising:
• inserting the speculum into a patient's vagina to expose the entire cervix of the patient;
• rinsing the patient's cervix with the saline solution contained in the first pre-filled container to remove discharge and detritus;
• visually identifying, using the light source and a colposcope, abnormal cervical vasculature with green light from the light source;
• applying the acetic acid contained in the second pre-filled container to the patient's cervix for staining abnormal tissue acetowhite;
• applying aqueous iodine solution to the patient's cervix for staining normal tissue brown;
• visualizing, using the colposcope, the patient's cervix and identifying brown staining of benign tissue in conjunction with acetowhite staining of abnormal tissue, and recording findings;
• rinsing the patient's cervix with the saline solution;
• applying the fluorescein sodium contained in the third pre-filled container to the patient's cervix;
• visualizing fluorescent green staining of malignant and premalignant tissue using blue light of the light source, and recording findings;
• rinsing the patient's cervix with the saline solution;
• performing one or more cervical biopsies and/or endocervical curettage to compare with cytological results;
• and
• if biopsies are performed and bleeding occurs at biopsy sites, obtaining hemostasis by applying silver nitrate to affected areas;
• and
• treating the cervical tissue indicating intraepithelial neoplasia based on a finding of the diagnosing, the treating comprising:
• applying trichloroacetic acid solution to an entirety of the patient's cervix;
• collecting, using a first swab, an amount of the applied trichloroacetic acid and further spreading the trichloroacetic acid solution to a squamocolumnar junction and an endocervix;
• removing, using a second swab, excess trichloroacetic acid solution to prevent incidental chemical cauterization of the vaginal wall;
• removing the speculum from the vagina;
• and
• repeating the diagnosing and treating until there are no findings of neoplastic tissue.