Back to results
US 10,588,320 B2Granted
Cell free supernatant composition of microbial culture for agricultural use
AssigneeConcentric AG Corporation
InventorsMargaret Bywater-Ekegard, Ananda Fitzsimmons
Life of this patent
Estimating the term…
Abstract
The present disclosure comprises compositions and methods for biostimulation, such as seed germination and plant growth. For example, the present disclosure comprises compositions and methods for affecting, enhancing, modulating, stimulating, or aiding plant growth. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
Claims12
A cell-free supernatant composition comprising:
- (i) an isolated cell-free supernatant of a microbial culture, obtained by (1) inoculating an isolated mixed microbial composition comprising isolated microorganisms of IN-M1, ATCC Patent Deposit Designation No. PTA-12383 into a culture medium comprising water, a molasses, a mineral powder, a sea salt, and a wheat bran, (2) incubating the inoculated media for 3-10 weeks at 30-32 or 35-37 or 32-37 degrees Celsius, thereby obtaining the microbial culture, and (3) centrifuging the microbial culture to separate microbial culture cells from a supernatant and filter sterilizing the supernatant to obtain the cell-free supernatant of the microbial culture, wherein the cell free supernatant comprises at least 2500 micrograms potassium per gram of cell-free supernatant, at least 435 micrograms nitrogen per gram of cell-free supernatant, at least 475 micrograms calcium per gram of cell-free supernatant, and at least 200 micrograms magnesium per gram of cell-free supernatant, and
- (ii) diluent water,
- wherein the ratio between the cell-free supernatant of the microbial culture and the diluent water in the cell-free supernatant composition is between 1:25 and 1:1000.
Dependencies are inferred from the claim text.