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US 10,588,298 B2Granted
Humanized IL-4 and IL-4Rα animals
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLi-Hsien Wang, Yingzi Xue, Andrew J. Murphy, Sean Stevens
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AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLi-Hsien Wang, Yingzi Xue, Andrew J. Murphy, Sean Stevens
Estimating the term…
Non-human animals comprising a human or humanized IL-4 and/or IL-4Rα nucleic acid sequence are provided. Non-human animals that comprise a replacement of the endogenous IL-4 gene and/or IL-4Rα gene with a human IL-4 gene and/or IL-4Rα gene in whole or in part, and methods for making and using the non-human animals, are described. Non-human animals comprising a human or humanized IL-4 gene under control of non-human IL-4 regulatory elements is also provided, including non-human animals that have a replacement of non-human IL-4-encoding sequence with human IL-4-encoding sequence at an endogenous non-human IL-4 locus. Non-human animals comprising a human or humanized IL-4Rα, gene under control of non-human IL-4Rα regulatory elements is also provided, including non-human animals that have a replacement of non-human IL-4Rα-encoding sequence with human or humanized IL-4Rα-encoding sequence at an endogenous non-human CIL-4Rα locus. Non-human animals comprising human or humanized IL-4 gene and/or IL-4Rα, sequences, wherein the non-human animals are rodents, e.g., mice or rats, are provided.
A rodent, comprising a replacement of a genomic DNA of a rodent IL-4Rα gene at an endogenous rodent IL-4Rα locus with a human genomic DNA of a human IL-4Rα gene to form a humanized IL-4Rα gene, wherein the genomic DNA of the rodent IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the rodent IL-4Rα gene, and the human genomic DNA of the human IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene, wherein the humanized IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene and exons 6-9 of the rodent IL-4Rα gene, wherein expression of the humanized IL-4Rα gene is under control of the rodent IL-4Rα promoter at the endogenous rodent IL-4Rα locus, and wherein the rodent is a mouse or a rat.
A rodent embryonic stem cell, comprising a replacement of a genomic DNA of a rodent IL-4Rα gene at an endogenous rodent IL-4Rα locus with a human genomic DNA of a human IL-4Rα gene to form a humanized IL-4Rα gene, wherein the genomic DNA of the rodent IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the rodent IL-4Rα gene, and the human genomic DNA of the human IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene, wherein the humanized IL-4Rα gene comprises the ATG initiation codon of exon 1 through exon 5 of the human IL-4Rα gene and exons 6-9 of the rodent IL-4Rα gene, wherein expression of the humanized IL-4Rα gene is under control of the rodent IL-4Rα promoter at the endogenous rodent IL-4Rα locus, and wherein the rodent embryonic stem cell is a mouse or rat embryonic stem cell.
A method for making a rodent, comprising
Dependencies are inferred from the claim text.