Aided delivery of plant treatment agents
AssigneeMonsanto Technology LLC
InventorsHuachun Larue, Fenggao Dong
Life of this patent
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Abstract
Provided herein are delivery methods of a plant treatment agent to a tissue of a plant, wherein the tissue is contacted with a plant treatment agent and delivery is aided by a centrifugal force applied to the plant. Disclosed herein is the discovery that drastic improvements in treatment delivery occur when plants are centrifuged after being contacted, submerged, unsubmerged, soaked, etc. in a solution containing a plant treatment agent. In one embodiment, haploid plants contacted with a colchicine solution are centrifuged to reveal improved chromosomal doubling rates as compared to control plants.
Claims19
A method of delivering a plant treatment agent to a cell of a selected tissue of a plant, wherein the plant has a surface, wherein the selected tissue is located at, and/or beneath, a portion of the plant surface, the method comprising contacting the plant surface with a solution that comprises the plant treatment agent, including contacting plant tissue other than the selected tissue, and applying a centrifugal force to the plant to transport the solution that comprises the plant treatment agent to the selected tissue,
- wherein the plant is supported inside a centrifuge container by a plant centrifugation support such that the shoot-root axis of the plant is aligned with the direction of the centrifugal force during centrifugation,
- wherein the plant centrifugation support comprises a structural support separate from the wall or walls of the centrifugation container,
- wherein the plant has germinated, and
- wherein the centrifugal force applied is from about 10 g to about 4,000 g,
- thereby delivering the plant treatment agent to the cell of the selected tissue of the plant.
A method of creating a doubled-haploid corn plant by delivering a chromosome doubling agent to a cell of a shoot meristem tissue, the method comprising contacting a DH0 mother plant with a solution that comprises the chromosomal doubling agent, including contacting plant tissue other than shoot meristem tissue, and applying a centrifugal force to the DH0 mother plant,
- wherein the plant is supported inside a centrifuge container by a plant centrifugation support such that the shoot-root axis of the plant is aligned with the direction of the centrifugal force during centrifugation,
- wherein the plant centrifugation support comprises a structural support separate from the wall or walls of the centrifugation container,
- thus delivering the solution that comprises the chromosome doubling agent to the cell of the shoot meristem tissue, wherein the corn plant has germinated.
A method for delivering a plant treatment agent to a cell of a selected tissue of a plant, wherein the plant has a surface, wherein the selected tissue is located at, and/or beneath, a portion of the plant surface, the method comprising the steps of:
- (a) contacting the surface of the plant with a solution comprising the plant treatment agent, wherein the plant has germinated;
- (b) applying a centrifugal force to the plant in step (a) contacted with the solution,
- wherein the plant is supported inside a centrifuge container by a plant centrifugation support such that the shoot-root axis of the plant is aligned with the direction of the centrifugal force during centrifugation,
- wherein the plant centrifugation support comprises a structural support separate from the wall or walls of the centrifugation container;
- (c) following the application of the centrifugal force in step (b), removing the plant from the centrifugal force and treatment solution;
- and
- (d) applying a subsequent centrifugal force to the plant subjected to the centrifugal force in step (b), thereby delivering the plant treatment agent to the plant tissue.
Dependencies are inferred from the claim text.