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US 10,586,692 B2Granted
Methods of multiplexing sample analysis by mass spectrometry
AssigneesThe Florida State University Research Foundation, Inc., St. Louis University
InventorsMichael G. Roper, James L. Edwards
Life of this patent
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Abstract
Provided herein are methods for multiplexed sample analysis by mass spectrometry. The methods may be performed without the need for chemical tagging. The methods also may include the analogous use of frequency modulation to multiplex mass spectrometric analysis, which may be referred to as frequency-modulated continuous flow analysis electrospray ionization mass spectrometry (FM-CFA-ESI-MS).
Claims17
A method of analysis, the method comprising:
- providing a first liquid at a first flow rate that is varied at a first frequency, wherein the first liquid comprises at least one first analyte;
- providing a second liquid at a second flow rate that is varied at a second frequency, wherein the second liquid comprises at least one second analyte, and wherein the first frequency and the second frequency are different;
- combining the first liquid and the second liquid with a third liquid to form a combined liquid, wherein the third liquid has a time-dependent velocity effective to impart the combined liquid with a substantially constant flow rate;
- analyzing the combined liquid with a mass spectrometer to monitor one or more selected m/z ratios;
- collecting one or more reconstructed ion chromatograms for the one or more selected m/z ratios;
- and
- analyzing the one or more reconstructed ion chromatograms to determine (i) which of the at least one first analyte, the at least one second analyte, or a combination thereof contributed to a signal, (ii) the relative concentration of the at least one first analyte, the at least one second analyte, or a combination thereof that contributed to the signal, or (iii) a combination thereof.
4 more elements
Dependencies are inferred from the claim text.