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US 10,584,320 B2Granted
Esterases and uses thereof
AssigneeCarbios
InventorsChristopher Topham, Helene Texier, Vincent Tournier, Marie-Laure Desrousseaux, Sophie Duquesne, Isabelle Andre, Sophie Barbe, Alain Marty
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel esterase, more particularly to esterase variants having improved thermostability compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
Claims20
An esterase variant which (i) has at least 75% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) has one or more amino acid modifications as compared to SEQ ID NO:
- 1 at position(s) selected from D203+5248, E173, L202, N204, F208, A172+A209, G39, A103, L82, G53, L104, L107, L119, A121, L124, I54, M56, L70, L74, A127, V150, L152, L168, V170, P196, V198, V200, V219, Y220, T221, S223, W224, M225, L239, T252, N253, H256, S1, Y4, Q5, R6, N9, S13, T16, S22, T25, Y26, S34, Y43, S48, T50, R72, S98, N105, R108, S113, N122, S145, K147, T160, N162, S181, Q189, N190, S193, T194, N204, S212, N213, N231, T233, R236, Q237, N241, N243, N254, R255 and Q258, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1, (iii) has a polyester degrading activity and (iv) exhibits increased thermostability as compared to the esterase of SEQ ID NO:
- 1.
1 more element
Dependencies are inferred from the claim text.