Back to results
US 10,577,635 B2Granted
Cell-free production of sugars
AssigneeGreenLight Biosciences, Inc.
InventorsDaniel MacEachran, Drew S. Cunningham, William Jeremy Blake, Matthew Eduardo Moura
Life of this patent
Estimating the term…
Abstract
Provided herein, in some embodiments, are systems, methods, and compositions (e.g., cells and cell lysates) for enzymatically converting a polymeric glucose carbohydrate (e.g., starch) to sugar.
Claims30
A method for producing allulose, the method comprising:
- converting fructose 6-phosphate (F6P) to allulose-6-phosphate (A6P), catalyzed by an allulose 6-phosphate epimerase (A6PE), wherein the A6PE is encoded by a nucleic acid sequence expressed in a microbial cell;
- and
- converting A6P to allulose, catalyzed by a Ruminiclostridium thermocellum allulose 6-phosphate phosphatase (A6PP).
A method for producing allulose, the method comprising:
- in a cell lysate mixture comprising cell lysate from microbial cells that express an allulose 6-phosphate epimerase (A6PE) and an allulose 6-phosphate phosphatase (A6PP), converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by the A6PE, and converting A6P to allulose, catalyzed by the A6PP,
- wherein the A6PP is encoded by an Ruminiclostridium thermocellum nucleic acid sequence.
A method comprising:
- in a cell lysate mixture, converting a polymeric glucose carbohydrate to allulose, wherein the cell lysate mixture comprises cell lysate from engineered microbial cells that express an α-glucan phosphorylase or a cellodextrin phosphorylase, a phosphoglucomutase, a phosphoglucoisomerase, an allulose 6-phosphate epimerase (A6PE), and an allulose 6-phosphate phosphatase (A6PP),
- wherein the A6PP is encoded by an Ruminiclostridium thermocellum nucleic acid sequence.
Dependencies are inferred from the claim text.