Polymers and DNA copolymer coatings
AssigneeIllumina Cambridge Limited
InventorsAndrew A. Brown, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Xavier von Hatten
Life of this patent
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Abstract
Some embodiments described herein relate to new polymer coatings for surface functionalization and new processes for grafting pre-grafted DNA-copolymers to surface(s) of substrates for use in DNA sequencing and other diagnostic applications.
Claims20
A substrate having a first surface comprising a polymer covalently bonded thereto, wherein the polymer comprises a recurring unit of Formula (IV):
- wherein each of R1c and R2c is independently hydrogen, optionally substituted alkyl, or optionally substituted phenyl;
- R3c is hydrogen, optionally substituted alkyl, optionally substituted phenyl, or optionally substituted C7-14 aralkyl;
- Ar is an optionally substituted C6-10 aryl or an optionally substituted 5 or 6 membered heteroaryl;
- RA is optionally substituted tetrazine;
- and
- L3 is a single bond, an optionally substituted alkylene linker, or an optionally substituted heteroalkylene linker.
A polymer for surface functionalization, wherein the polymer comprises a recurring unit of Formula (IV):
- wherein each of R1c and R2c is independently hydrogen, optionally substituted alkyl, or optionally substituted phenyl;
- R3c is hydrogen, optionally substituted alkyl, optionally substituted phenyl, or optionally substituted C7-14 aralkyl;
- Ar is an optionally substituted C6-10 aryl or an optionally substituted 5 or 6 membered heteroaryl;
- RA is optionally substituted tetrazine;
- and
- L3 is a single bond, an optionally substituted alkylene linker, or an optionally substituted heteroalkylene linker.
A method for immobilizing a polymer to a first surface of a substrate, comprising:
- contacting a polymer comprising a recurring unit of Formula (IV) with the first surface of the substrate, said first surface comprising a first plurality of functional groups covalently attached thereto;
- and
- reacting the first plurality of functional groups of the first surface with the polymer, thereby covalently bonding the polymer to the first surface of the substrate;
- wherein each of R1c and R2c is independently hydrogen, optionally substituted alkyl, or optionally substituted phenyl;
- R3c is hydrogen, optionally substituted alkyl, optionally substituted phenyl, or optionally substituted C7-14 aralkyl;
- Ar is an optionally substituted C6-10 aryl or an optionally substituted 5 or 6 membered heteroaryl;
- RA is optionally substituted tetrazine;
- and
- L3 is a single bond, an optionally substituted alkylene linker, or an optionally substituted heteroalkylene linker.
Dependencies are inferred from the claim text.