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OwnerMarc Allan Eichmeyer, Gregory Paul Nitzel +1inventors
InventorsMarc Allan Eichmeyer, Gregory Paul Nitzel, Merrill Lynn Schaeffer
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An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
A kit comprising a container, wherein said container comprises:
A kit comprising a container, wherein said container comprises:
A method for aiding in the prevention or reduction of one or more of clinical symptoms associated with PCV2 infection, wherein the clinical symptoms are selected from the group consisting of PCV2 virus shedding, lymphoid infection caused by PCV2, increased mortality rate, decreased average daily weight gain, PCV2 viremia, and any combination thereof in pigs, said method comprising administering a single dose of a recombinantly produced PCV2 ORF2 antigen to a pig.
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