Compositions comprising a polypeptide having cellulolytic enhancing activity and a heterocyclic compound and uses thereof

An enzyme composition comprising a glycoside hydrolase family 61 (GH61) polypeptide having cellulolytic enhancing activity and a heterocyclic compound, wherein the combination of the GH61 polypeptide having cellulolytic enhancing activity and the heterocyclic compound enhances hydrolysis of a cellulosic material by the enzyme composition compared to the GH61 polypeptide having cellulolytic enhancing activity alone or the heterocyclic compound alone with a ratio greater than 1 according to the following formula:

• GH61 effect=% conversion(+GH61+heterocyclic compound)/% conversion(no GH61+heterocyclic compound)
• wherein the heterocyclic compound is a compound of formula (I) or (II):
• wherein each bond indicated with a dashed line is single or double;
• R1, R2, R3, R4, and R5 are independently hydrogen, halogen, ═O, —OH, —OR8, —CN, —NO2, —N(R9)(R10), —C(O)R20, —C(O)OR6, —C(O)NHR7, —OC(O)R11, —NHC(O)R12, —OC(O)OR13, —NHC(O)OR14, —OC(O)NHR15, —NHC(O)NHR16, —SO2R17, —SO2N(R18)(R19), —SR21, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
• R6, R7, R8, R9, R19, R11, R12, R13, R14, R15, R16, R18, R19, R20, and R21 are independently hydrogen, or an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
• and
• R17 is an optionally substituted moiety selected from alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkyl-alkyl, heterocycloalkyl, heterocycloalkyl-alkyl, aryl, aralkyl, heteroaryl, and heteroaralkyl;
• and
• wherein each pair of R1 and R2, R2 and R3, R3 and R4, and R4 and R5 may combine to form an optionally substituted fused ring;
• or a salt or solvate thereof.