TC-83-derived alphavirus vectors, particles and methods
AssigneeAlphaVax, Inc.
InventorsJon O. Rayner, Jonathan F. Smith, Bolyn Hubby, Elizabeth A. Reap
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Abstract
The present disclosure provides TC-83 VEE-derived replicons, alphaviral replicon particles and immunogenic compositions containing TC-83 alphaviral replicon particles which direct the expression of at least one antigen when introduced into a suitable host cell. The TC-83 VEE-derived ARPs described herein are improved in that they are subject to a lower vector-specific immune response than prior art ARPs.
Claims14
A helper cell for producing infectious, propagation-defective alphavirus particles comprising:
- (a) an alphavirus replicon RNA encoding at least one heterologous RNA sequence and lacking sequences encoding alphavirus structural proteins;
- and
- (b) (i) a first helper RNA encoding at least one, but not all, Venezuelan equine encephalitis virus TC83 structural proteins, and (ii) a second helper RNA encoding all of the Venezuelan equine encephalitis virus TC83 structural proteins required to produce infectious, propagation-defective TC-83 replicon particles in the cell not encoded by the first helper RNA;
- or
- (iii) one or more helper DNA plasmids encoding all of the Venezuelan equine encephalitis virus TC83 structural proteins.
A helper cell for producing infectious, propagation-defective alphavirus particles comprising:
- (a) an alphavirus replicon RNA encoding at least one heterologous RNA sequence and lacking sequences encoding alphavirus structural proteins;
- and
- (b) (i) a first helper RNA encoding at least one, but not all, Venezuelan equine encephalitis virus TC83 structural proteins, and (ii) a second helper RNA encoding all of the Venezuelan equine encephalitis virus TC83 structural proteins required to produce infectious, propagation-defective TC-83 replicon particles in the cell not encoded by the first helper RNA.
Dependencies are inferred from the claim text.