Targeted histone acetylation
AssigneeSigma-Aldrich Co.
InventorsQingzhou Ji, Carol Kreader
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Abstract
The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
Claims4
A fusion protein comprising a DNA binding domain and a domain having histone acetyltransferase activity that consists essentially of amino acids 1100-1750 of SEQ ID NO:13 or amino acids 1284-1673 of SEQ ID NO:13, wherein the DNA binding domain is a zinc finger DNA binding domain comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:9 and SEQ ID NO:10.
Dependencies are inferred from the claim text.