Multi-enzyme conjugate, method for preparing the same and method for preparing organic compound using the same
AssigneeGwangju Institute of Science and Technology
InventorsInchan Kwon, Jinhwan Cho, Sung In Lim
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Abstract
The present disclosure relates to a multi-enzyme conjugate, a method for preparing the same and a method for preparing an organic compound using the same. More particularly, a multi-enzyme conjugate exhibiting improved catalytic efficiency over respective free enzymes using site-specific incorporation of a clickable non-natural amino acid into the enzymes and two compatible click reactions, a method for preparing the same and a method for preparing an organic compound using the same may be provided.
Claims2
A method for synthesizing an organic compound, comprising a step of performing a multi-enzyme cascade reaction using a multi-enzyme conjugate, wherein
- the multi-enzyme conjugate has the following structure:
- where FDH is a formate dehydrogenase obtained from Thiobacillus sp. KNK65MA, and MDH is a mannitol-2-dehydrogenase obtained from Pseudomonas fluorescens,
- the multi-enzyme cascade reaction comprises a first enzymatic reaction and a second enzymatic reaction,
- a product of the first enzymatic reaction is used as a reactant of the second enzymatic reaction,
- the first enzyme of the multi-enzyme conjugate acts as a biocatalyst of the first enzymatic reaction, and
- the second enzyme of the multi-enzyme conjugate acts as a biocatalyst of the second enzymatic reaction.
Dependencies are inferred from the claim text.