Methods for producing abienol
AssigneeDsm IP Assets B.V.
InventorJohn Royer
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Abstract
The present disclosure relates to a novel method, expression vectors, and host cells for producing abienol by converting geranylgeranyl diphosphate (GGPP) to abienol in the presence of a combination of a class II diterpene synthase and a bifunctional class I/II abienol synthase.
Claims11
A method for producing abienol, which comprises converting geranylgeranyl diphosphate (GGPP) to abienol by contacting the GGPP with a combination of class II diterpene synthase (diTPS) and bifunctional class I/II abienol synthase (CAS) whereby the class II diTPS converts the GGPP to labdenediol diphosphate and the bifunctional CAS converts the GGPP to labdenediol diphosphate and the labdenediol diphosphate to abienol, wherein the class II diTPS comprises an amino acid sequence with at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:2 and the bifunctional CAS comprises an amino acid sequence with at least 95% sequence identity to SEQ ID NO:3.
Dependencies are inferred from the claim text.