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US 10,562,004 B2Granted
Method of fabricating cell arrays and uses thereof
AssigneeStemoniX Inc.
InventorRobert John Petcavich
Life of this patent
Estimating the term…
Abstract
The present disclosure provides a fabrication process that results in creating large arrays of living cells, such as stem cells, which are subsequently exposed to nanoliter quantities of compounds to test the efficacy on cellular metabolism.
Claims19
A method of fabricating and dosing a viable cell array, comprising:
- providing a mixture of human cells and a UV curable hydrogel matrix, wherein the human cells are embryonic stem cells, induced pluripotent stem cells (iPSC) or cardiomyocytes;
- applying a volume of the mixture to a support, in a specific geometry, to form a N×N array, wherein the substrate comprises a preembossed film substrate having microstructures that provide for the specific geometry, wherein the microstructures are coated with one or more cellular adhesion polymers, and wherein the support is laminated to a microplate;
- exposing the applied volume to UV light to cure each volume in the array and incubating the array under conditions that provide for cell viability;
- contacting each volume of cells in the array with a volume of the one or more compounds using ultrasonic deposition, wherein a nanoliter dispensing system provides the volume of the one or more compounds;
- and
- detecting whether the one or more compounds affects the viable cells in the array.
3 more elements
A method of fabricating a stem cell array, comprising:
- mixing human induced pluripotent stem cells (iPSC) into a UV curable hydrogel matrix comprising gelatin;
- applying the mixture onto a support to provide a N×N array of the hydrogel stem cell mixture, wherein a mold or microstructures in the array provide for a specific geometry;
- and
- applying ITV light to cure the mixture in the presence of a micro mold template.
1 more element
Dependencies are inferred from the claim text.