Expression of thermostable starch synthase genes improves the yield in heat stress
AssigneeKansas State University Research Foundation
InventorsHarold N. Trick, Allan Fritz, Shyamal Talukder
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Abstract
Genetically-modified plants having increased tolerance to heat stress are described. Methods of producing such genetically-modified plants are also disclosed. The genetically-modified plants comprise exogenous nucleic acid encoding a thermostable protein having starch synthase activity. Genetically-modified plants have increased yield when grown under elevated conditions as compared to control plants.
Claims16
A genetically-modified plant having increased tolerance to heat stress as compared to a control plant, wherein said genetically-modified plant comprises a heterologous exogenous nucleic acid encoding soluble starch synthase from grape or cottonwood, wherein said soluble starch synthase from grape or cottonwood is expressed in said genetically-modified plant, said exogenous nucleic acid comprising a nucleotide sequence of SEQ ID NO:3 or 5, or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 4 or 6.
A method of increasing tolerance to heat stress in a plant, said method comprising:
- transforming said plant with a heterologous exogenous nucleic acid encoding soluble starch synthase from grape or cottonwood, wherein said soluble starch synthase is expressed in said genetically-modified plant to yield a transformed plant, thereby increasing the heat stress tolerance of said transformed plant, said exogenous nucleic acid comprising a nucleotide sequence of SEQ ID NO:3 or 5, or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 4 or 6.
A recombinant plant cell having ectopic expression of a heterologous exogenous soluble starch synthase from grape or cottonwood by stable transformation with a nucleic acid construct encoding said soluble starch synthase, wherein said nucleic acid is synthesized from a native soluble starch synthase gene isolated from grape or cottonwood and cloned into said construct, said exogenous nucleic acid comprising a nucleotide sequence of SEQ ID NO:3 or 5, or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:
- 4 or 6.
A codon optimized cDNA sequence for transforming plants for increased heat tolerance selected from the group consisting of SEQ ID NO:3 and 5.
Dependencies are inferred from the claim text.