Selection marker for cell transfection and protein production
AssigneeSanofi
InventorsBruno Dumas, Nabil Lounis
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Abstract
The present invention is within the field of industrial protein production. The invention provides a novel expression system using dihydroorotate dehydrogenase (DHODH) as a selection marker in combination with leflunomide or a metabolite thereof, notably for use in mammalian cell lines. Expression vectors encoding DHODH, cell lines comprising said vectors and methods of producing recombinant proteins are also provided.
Claims20
An expression vector comprising the nucleotide sequence of SEQ ID NO:3 encoding a mammalian dihydroorotate dehydrogenase (DHODH) and at least one expression cassette for expressing a recombinant protein, wherein the DHODH comprises the amino acid sequence of SEQ ID NO:4, wherein the DHODH is inhibited by at least one DHODH inhibitor.
Dependencies are inferred from the claim text.