Methods and compositions for improving plant traits
AssigneePivot Bio, Inc.
InventorsKarsten Temme, Alvin Tamsir, Sarah Bloch, Rosemary Clark, Emily Tung
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Abstract
Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.
Claims17
A method of increasing an amount of atmospheric derived nitrogen in a corn plant in a field, the method comprising:
- exposing a corn plant in a field to a plurality of engineered diazotrophs comprising at least one genetic variation introduced in a nitrogen fixation genetic regulatory network that increases expression or activity of nifH, wherein the at least one introduced genetic variation comprises genetic material that originates from at least one organism of the same genus as said plurality of engineered diazotrophs,
- whereby the corn plant exposed to the engineered diazotrophs comprises an increased amount of atmospheric derived nitrogen relative to a corn plant exposed to the same amount of non-engineered diazotrophs of the same species as the engineered diazotrophs.
Dependencies are inferred from the claim text.