Nanocarriers and their processing for diagnostics and therapeutics
AssigneeRhode Island Hospital
InventorsQian Chen, Yupeng Chen, Hongchuan Yu, Michael G. Ehrlich
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Abstract
The compositions and methods of the invention provide compositions and methods for preferential targeting of tissues to delivery therapeutic or diagnostic agents. For example, such compounds are useful in the treatment of joint disorders those affecting articulating joints, e.g., injury-induced osteoarthritis as well as autoimmune diseases affecting joint tissue such as rheumatoid arthritis.
Claims32
A method of treating joint disease comprising administration to a subject of an effective amount of a nanopiece, wherein said nanopiece comprises i) a compound of Formula I or Formula II or a combination thereof, and ii) a nucleic acid:
- wherein, X is CH or N;
- R2 is hydrogen or a linker group;
- Y is absent when R2 is hydrogen or is an amino acid side chain, amino acid or polypeptide;
- and
- R1 is hydrogen or aliphatic, wherein the nanopiece has a size in at least one dimension between 0.1 nm and 150 nm,
- wherein a ratio of the compound to nucleic acid ranges from 4.4 to 30 μg compound per to 0.1 nmol of the nucleic acid;
- wherein the nanopiece is positively charged at pH 7-7.5.
A method of treating a joint disease comprising
- (i) providing a cell from a subject;
- (ii) introducing siRNA into a cell by contacting the cell with a nanopiece comprising the siRNA and a compound of Formula I or Formula II or a combination thereof,
- wherein, X is CH or N;
- R2 is hydrogen or a linker group;
- Y is absent when R2 is hydrogen or is an amino acid side chain, amino acid or poly peptide;
- and
- R1 is hydrogen or aliphatic;
- and
- (iii) reintroducing the cell into the subject,
- wherein the nanopiece has a size in at least one dimension between 0.1 nm and 150 nm,
- wherein a ratio of the compound to the siRNA ranges from 4.4 to 30 μg compound per to 0.1 nmol of the siRNA;
- wherein the nanopiece is positively charged at pH 7-7.5.
Dependencies are inferred from the claim text.