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US 10,551,382 B2Granted
Enzyme quantification
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren Roy Link, Michael L. Samuels
Life of this patent
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Abstract
The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
Claims15
A method for identifying components of a chemical reaction, the method comprising:
- forming a plurality of fluid partitions each comprising a maximum of one particle that comprises a marker and a fluorescently labeled binder specific to the marker;
- incubating the fluid partitions, wherein the fluorescently labeled binder binds to the particle comprising the marker in at least one of the fluid partitions;
- and
- quantifying the particles by determining a number of fluid partitions each comprising a localized signal from the labeled binder on the particle.
1 more element
Dependencies are inferred from the claim text.