Methods for increasing the capacity of flow-through processes
AssigneeEMD Millipore Corporation
InventorsVenkatesh Natarajan, Herbert Lutz, Bala Raghunath
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Abstract
In various embodiments, the present invention provides a process for separating target proteins from non-target proteins in a sample comprising increasing the concentration of the target proteins and non-target proteins in the sample and subsequently delivering the concentrated sample to a chromatography device. In other embodiments, the invention relates to a process for increasing the capacity of a chromatography device for a target protein by delivering a concentrated sample comprising the target protein to a chromatography device.
Claims16
A process for separating target proteins from non-target proteins, comprising:
- a) obtaining a liquid sample containing target proteins and non-target proteins;
- b) increasing the concentration of the target proteins and non-target proteins in the sample by an ultrafiltration process to produce a concentrated feed for delivery to a chromatography device, the ultrafiltration process being a recirculating tangential flow filtration (TFF) process, a single-pass TFF (SPTFF) process, or a TFF process operated in a single-pass TFF mode;
- c) delivering the concentrated feed to the chromatography device, the chromatography device comprising an affinity chromatography medium;
- d) separating the target proteins from the non-target proteins in the chromatography device;
- and
- e) recovering the target proteins from the chromatography device in a recovered sample;
- wherein the steps:
- a)-e) are performed in order.
A process for increasing the capacity of a chromatography device for a target protein, comprising:
- a) obtaining a liquid sample containing target proteins and non-target proteins;
- b) increasing the concentration of the target proteins and non-target proteins in the sample by an ultrafiltration process to produce a concentrated feed for delivery to a chromatography device, the ultrafiltration process being a recirculating tangential flow filtration (TFF) process, a single-pass TFF (SPTFF) process, or a TFF process operated in a single-pass TFF mode;
- and
- c) delivering the concentrated feed to the chromatography device, the chromatography device comprising an affinity chromatography medium, wherein the capacity of the chromatography device for the target protein is increased;
- wherein the steps:
- a)-c) are performed in order.
A process for filtering viruses from a liquid sample, comprising:
- a) obtaining a liquid sample containing viruses;
- b) increasing the concentration of the target proteins and viruses in the sample by an ultrafiltration process to produce a concentrated feed for delivery to a viral filtration device, the ultrafiltration process being a recirculating tangential flow filtration (TFF) process, a single-pass TFF (SPTFF) process, or a TFF process operated in a single-pass TFF mode, a TFF device of the ultrafiltration process being coupled to the viral filtration device;
- c) delivering the concentrated feed from the TFF device to the viral filtration device;
- and
- d) removing viruses from the feed in the viral filtration device;
- wherein the steps:
- a)-d) are performed in order.
Dependencies are inferred from the claim text.