Multiplexed biological assay device with electronic readout
AssigneeLucira Health, Inc.
InventorsFrank B. Myers, III, Clay D. Reber, Taber H. Smith, Faisal S. Maniar
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Abstract
This invention relates generally to devices, systems, and methods for performing biological assays by using indicators that modify one or more optical properties of the assayed biological samples. The subject methods include generating a reaction product by carrying out a biochemical reaction on the biological sample introduced into a device and reacting the reaction product with an indicator capable of generating a detectable change in an optical property of the biological sample to indicate the presence, absence, or amount of analyte suspected to be present in the sample.
Claims126
An assembly for performing a biological assay, the assembly comprising:
- a first piece comprising a first face;
- and
- a second piece comprising a second face,
- wherein the first piece and the second piece are operatively coupled to create a plurality of independent, continuous fluidic pathways, the continuous fluidic pathways comprising:
- a common sample receiving inlet;
- a plurality of fluid channels extending from and in fluidic communication with the common sample receiving inlet;
- and
- a plurality of fluidic chambers, each fluidic chamber substantially equidistant from a single sensing region, and each fluidic chamber comprising a fluid inlet in fluidic communication with a terminus of one of the plurality of fluid channels and an outlet vent at a fluidic pathway terminus,
- wherein the second piece comprises a transparent material forming a first plurality of light pipes, each first light pipe capable of transmitting light between one of the plurality of fluidic chambers and the single sensing region.
A system for performing a biological assay, the system comprising:
- an assembly, the assembly comprising:
- a first piece comprising a first face;
- a second piece comprising a second face,
- wherein the first piece and the second piece are operatively coupled to create a plurality of independent, continuous fluidic pathways, the continuous fluidic pathways comprising:
- a common sample receiving inlet;
- a plurality of fluid channels extending from and in fluidic communication with the common sample receiving inlet;
- and
- a plurality of fluidic chambers, each fluidic chamber substantially equidistant from a single sensing region, and each fluidic chamber comprising a fluid inlet in fluidic communication with a terminus of one of the plurality of fluid channels, and an outlet vent at a fluidic pathway terminus, the outlet vent sealed by a material,
- wherein the second piece comprises a transparent material forming a first plurality of light pipes, each first light pipe capable of transmitting light between one of the plurality of fluidic chambers and the single sensing region;
- and
- a circuit board operatively coupled to the fluidic chambers, the circuit board comprising:
- a microprocessor;
- a plurality of light emitting elements, each light emitting element capable of individually illuminating one of the plurality of fluidic chambers;
- a photosensor optically coupled to the single sensing region;
- a heating element comprising a ring shape, the heating element configured to heat the plurality of fluidic chambers;
- a temperature sensor;
- and
- an electronic result display mechanism,
- wherein the microprocessor is programmed to cause each of the plurality of light emitting elements to emit light in a repeating pattern at a repetition frequency, wherein only one of the plurality of fluidic chambers is illuminated at any time, and wherein the microprocessor is further programmed to analyze signals received from the photosensor, to generate signals transmitted to the heating element, to analyze signals received from the temperature sensor, and to generate signals transmitted to the electronic result display mechanism.
Dependencies are inferred from the claim text.