Method for preparing broccoli protein peptide mixture
AssigneesZhejiang Hisun Pharmaceutical Co., Ltd., Zhejiang Teley Biotechnology Co., Ltd.
InventorsJidong Wang, Huan Qi, Xiaohe Zheng, Hui Zhang, Yongdong Wang, Hailing Tang, Haiming Lin
Life of this patent
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Abstract
Provided is a method for preparing a broccoli protein peptide. The method uses a broccoli protein as the raw material, and obtains a broccoli protein peptide powder through the steps of preprocessing, enzymatic hydrolysis, terminating enzymatic hydrolysis, separation, and drying and the like. Also provided is the use of the prepared broccoli protein peptide in resisting oxidation, reducing cholesterol and lowering blood lipids.
Claims10
A method for preparing a broccoli protein peptide mixture, the method comprising:
- (a) adding water, anhydrous sodium sulfite, and EDTA to a raw material of broccoli protein thereby making a predigestion mixture,
- wherein the weight of the water is 4 to 8 times that of raw material of broccoli protein, the EDTA has a mass/volume ratio of 0.02-0.05 g/L, and the anhydrous sodium sulfite has a mass/volume ratio of 0.05-0.1 g/L;
- (b) digesting the predigestion mixture for 3-4 hours at a temperature with an enzyme selected from the group consisting of neutral protease, papain, alkaline protease, trypsin, pepsin, bromelain and any combination thereof, thereby forming a digested mixture;
- (c) terminating the digestion by heating the digested mixture to from 80-90° C. for from 5-15 minutes;
- (d) optionally, centrifuging or filtering the mixture from (c);
- (e) filtering the liquid from (c) or (d) with a membrane having a pore size of 100 to 500 nm;
- (f) optionally, debittering the mixture from (e) by treating with active carbon or clay;
- and
- (g) concentrating and/or drying the mixture from (e) or (f), thereby obtaining the broccoli protein peptide mixture.
Dependencies are inferred from the claim text.