# US 10,548,853 B2 · Oncolytic virus formulation and preparation method thereof

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- Number: US 10,548,853 B2
- Document ID: US10548853B2
- Status: Granted
- Kind: B2
- Application number: 15/825,065
- Filed: 2017-11-28
- Published: 2018-03-22
- Granted: 2020-02-04
- Earliest claimed: 2015-05-29
- Assignees: Hubei Soundny Biotechnology Co., Ltd.
- Applicants: Hubei Soundny Biotechnology Co., Ltd.
- Inventors: Bo Huang
- Web page: https://patentgrep.com/patent/US10548853B2

## CPC

- A61K 9/5068: {Cell membranes or bacterial membranes enclosing drugs (liposomes with additional exogenous lipids A61K9/127)}
- A61K 9/0019: {Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner (non-active ingredients are additionally classified in A61K47/00)}
- A61K 9/5089: {Processes}
- A61K 35/13: Tumour cells, irrespective of tissue of origin (tumour vaccines A61K39/00)
- A61K 35/76: Viruses; Subviral particles; Bacteriophages
- A61K 35/761: Adenovirus
- A61P 35/00: Antineoplastic agents
- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- C12N 7/02: Recovery or purification
- C12N 2710/10332: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2710/10351: Methods of production or purification of viral material
- C12N 2720/12032: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2760/20232: Use of virus as therapeutic agent, other than vaccine, e.g. as cytolytic agent
- C12N 2760/20251: Methods of production or purification of viral material
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

The present invention provides an oncolytic virus formulation and preparation method thereof. The oncolytic virus formulation comprises cell vesicles derived from apoptotic tumor cells and oncolytic viruses coated in the cell vesicles as an effective component. The oncolytic virus formulation uses cell vesicles derived from tumor cells themselves to coat the oncolytic viruses, so as to evade the body's immune system attack and can be targeted to the tumor treatment site, and improve the tumor-killing effect.

## Claims

### Claim 1

A method for preparing an oncolytic virus formulation, comprising mixing oncolytic viruses and tumor cells in a ratio of 1:1 to 20:1 so as to infect tumor cells, and culturing the infected tumor cells to induce apoptosis at 37° C. and 5% oxygen content, collecting microparticles released from the apoptotic tumor cells within 48-72 hours, wherein the microparticles are the oncolytic virus formulation generated after the oncolytic virus is coated with cell vesicles;
wherein the collection of the microparticles released from the apoptotic tumor cells comprises removing the tumor cells and debris by an ultracentrifuge at a centrifugal force of not more than 5000 g, and collecting the microparticles by the ultracentrifuge at a centrifugal force of 10000 g.

### Claim 2 (depends on 1)

The method according to claim 1, wherein the tumor cells include cells of ovarian cancer, breast cancer, lung cancer, gastric cancer, colon cancer, liver cancer, pancreatic cancer or prostate cancer.