Fungal strain Beauveria sp. MTCC 5184 and a process for the preparation of enzymes therefrom

A composition comprising:

• a protease enzyme and an organic solvent, wherein the protease enzyme is from a Beauveria species bearing accession number MTCC 5184, and the organic solvent comprises acetone, ethanol, isopropanol, methanol, butanol, chloroform, dimethyl sulfoxide (DMSO), or hexane;
• wherein the composition is prepared by a process comprising:
• a) culturing the Beauveria species in a medium comprising 0.15 to 80% of a carbon source, 0.15 to 80% of a nitrogen source, and an inducer, wherein the carbon and nitrogen sources are optionally inducers under aerobic conditions at a pH ranging from 5.0 to 9.0 and a temperature ranging between 15° to 32° C. for a period ranging between 2 to 7 days;
• b) harvesting the medium obtained in step (a);
• c) separating/extracting the protease enzyme from the harvested medium in liquid phase;
• and
• d) providing the protease enzyme obtained in step (c) with the organic solvent;
• wherein the protease enzyme in the composition has a greater residual activity (%) than a control sample comprising an identical concentration of the protease enzyme in water, after 1 hour at 50° C.;
• and
• wherein the protease enzyme is active in a pH range of 5.5 to 12, provides its maximum activity at 50° C., and is stable in a pH range of 3 to 11.