Viral vector manufacture
AssigneeSementis Limited
InventorsPaul Michael Howley, Liang Liu
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Abstract
This present invention relates to a modified mammalian cell in which the genome of the cell is modified to comprise a sequence encoding CP77 under the control of a promoter such that the modified cell line sustains propagation of a poxvirus that is less able or unable to propagate in the unmodified cell.
Claims9
A modified Chinese Hamster Ovary (CHO) cell in which the genome of the CHO cell is modified to comprise a sequence encoding CP77 under the control of a constitutive promoter such that the modified CHO cell line sustains propagation of a vaccinia virus and wherein the genome of the modified CHO cell further comprises a sequence encoding D13L under the control of a constitutive promoter and/or a sequence encoding K1L under the control of a constitutive promoter;
- with the proviso that CP77, D13L and K1L do not include functional orthologs and functional variants.
A process for propagating a vaccinia virus in CHO cells, the process comprising propagating the vaccinia virus in vitro in a Chinese Hamster Ovary (CHO) cell wherein the CHO cell is modified to encode and express CP77 under the control of a constitutive promoter and to encode and express D13L under the control of a constitutive promoter;
- with the proviso that CP77 and D13L do not include functional orthologs and functional variants.
Dependencies are inferred from the claim text.