Modified-dextrans for use in optical glucose assays
AssigneeMedtronic MiniMed, Inc.
InventorsTri T. Dang, Soren Aasmul, Jesper Svenning Kristensen, Joseph Hanna, Robert McKinlay
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Abstract
The invention is directed to a competitive glucose binding affinity assay comprising a glucose receptor (typically mannan binding lectin) labeled with an assay fluorophore and a modified glucose analog (typically dextran) labeled with a reference fluorophore. In certain embodiments, the glucose analog is dextran and is coupled to both a reference fluorophore and a quencher dye (e.g. hexamethoxy crystalviolet-1). Optionally the reference fluorophore is blue shifted relative to the assay fluorophore.
Claims13
A glucose sensing complex comprising:
- mannan binding ligand, wherein the mannan binding ligand is coupled to an assay fluorophore;
- a reference fluorophore;
- dextran, wherein the dextran functions in the assay as a glucose analog and is coupled to:
- the reference fluorophore;
- an agent that enhances the hydrophilicity of the dextran;
- a quenching agent, wherein the assay fluorophore and quenching agent form a Förster Resonance Energy Transfer (FRET) pair;
- and
- the glucose sensing assay complex exhibits a sensor dose response (DR) loss of less than 2.5% per day.
A glucose sensing complex comprising:
- mannan binding ligand, wherein the mannan binding ligand is coupled to an assay fluorophore;
- a reference fluorophore;
- dextran, wherein the dextran functions in the assay as a glucose analog and is coupled to:
- the reference fluorophore;
- an agent that enhances the hydrophilicity of the dextran, wherein the agent that enhances the hydrophilicity of the dextran comprises a cyclic anhydride, a tartaric anhydride derivate, a succinic acid, a glutaric acid, an adipic acid, a pimelic acid, a suberic acid, an azelaic acid or a sebacic acid;
- and
- a quenching agent, wherein the assay fluorophore and quenching agent form a Förster Resonance Energy Transfer (FRET) pair.
Dependencies are inferred from the claim text.