Multiview light-sheet microscopy

A microscope system for imaging of a live biological specimen, the system comprising:

• a specimen region configured to receive a biological specimen;
• three or more optical arms, each optical arm having an optical path that crosses the specimen region, each optical arm comprising a light source unit and a detection unit, and each optical arm arranged along a distinct arm axis, at least two arm axes being not parallel with each other;
• wherein the light source unit in each optical arm is distinct from the light source unit in each of the other optical arms;
• each light source unit comprising a light source and a set of illumination optical devices arranged to produce and direct a light sheet toward the specimen region along a respective illumination axis, and a set of actuators coupled to one or more illumination optical devices;
• each detection unit comprising a camera and a set of detection optical devices arranged to collect and record images of fluorescence emitted from a biological specimen received in the specimen region along a respective detection axis that is perpendicular to one or more of the illumination axes, and a set of actuators coupled to one or more of the camera and the detection optical devices;
• and
• each optical arm including an optical data separation apparatus on the path between the specimen region and the light source unit and the detection unit and configured to separate optical data between the light source unit and the detection unit.