PIVKA-II measurement method, measurement reagent, and measurement kit
AssigneesFujirebio Inc., Sekisui Medical Co., Ltd.
InventorsKentaro Yamaguchi, Katsumi Aoyagi, Azusa Terao
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Abstract
Disclosed are a PIVKA-II measurement method that achieves better serum-plasma correlation than conventional methods, and a reagent and a kit therefor. The PIVKA-II measurement method according to the present invention comprises measuring PIVKA-II in a sample by an immunoassay using a mixture of an anti-F1 antibody that specifically binds to prothrombin fragment F1 or an antigen-binding fragment thereof and an anti-F2 antibody that specifically binds to prothrombin fragment F2 or an antigen-binding fragment thereof; and an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof.
Claims6
A method for measuring Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II), comprising:
- measuring PIVKA-II in a sample by carrying out an immunoassay using antibodies comprising (a) an anti-PIVKA-II antibody that specifically binds to PIVKA-II or an antigen-binding fragment thereof and (b) a mixture of (i) an anti-F1 antibody that specifically binds to prothrombin fragment 1 or an antigen-binding fragment thereof and (ii) an anti-F2 antibody that specifically binds to prothrombin fragment 2 or an antigen-binding fragment thereof,
- wherein said anti-F1 antibody, said anti-F2 antibody, and said anti-PIVKA-II antibody are monoclonal antibodies,
- wherein the mixture of the anti-F1 antibody and the anti-F2 antibody is in a mixing ratio of 1:0.2-2,
- wherein the immunoassay is a sandwich immunoassay using said mixture as a labeled antibody and said anti-PIVKA-II antibody as an immobilized antibody, or using said mixture as an immobilized antibody and said anti-PIVKA-II antibody as the labeled antibody, and
- wherein said sample is serum or plasma.
Dependencies are inferred from the claim text.