High-throughput imaging platform

A microfluidic device comprising:

• two or more substrates bonded together to form a plurality of trapping devices, each trapping device comprising:
• a sample reservoir;
• an inlet channel in fluid communication with the sample reservoir;
• a plurality of trapping channels in fluid communication with the inlet channel, each trapping channel having a length, a width, and a height;
• and
• an exit channel in fluid communication with the plurality of trapping channels, wherein:
• the length of each trapping channel is measured in a direction from the inlet channel to the exit channel through the trapping channel, the width is measured in a direction orthogonal to the length direction and within a plane that is parallel to the surface of at least one of the substrates, and the height is measured in a direction that is orthogonal to the length and width directions,
• each trapping channel comprises a plurality of sections arranged end to end along the length of the respective trapping channel, wherein the widths of at least two adjacent sections vary with respect to each other,
• for at least one trapping channel, the heights of at least two of the plurality of sections for trapping a biological subject differ from each other such that the aspect ratio of the width to the height of the respective section is within a range of about 0.2 to about 2.0, and
• the exit channels of at least two of the plurality of trapping devices are in fluid communication with one another via an intermediate exit channel.